Indicators on AGX51 You Should Know
The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR solutions had been digested and cloned into a pET21a+ vector using NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the quick alter web page-directed mutagenesis method uti